The year is 2046. Detective Ross Sylibus is transferred to Mars when a country singer on her flight is murdered. Making matters more complicated is that the singer is a "Third"—a robot that looks and feels like a human. Sylibus is partnered with Armitage—a beautiful female cop with a bad attitude. As they investigate the murder of the singer and other women on Mars, they uncover a conspiracy that can have them both killed by the Martian government.

Set in a different series of events, this story does not follow the previous Appleseed movie canon (despite being directed by the same director of the previous movies); it is an alternate story to their beginnings. Briareos is already a cyborg and did not become separated from Deunan to be later reunited in Olympus to join ESWAT.

Appleseed Alpha depicts the early days of Deunan Knute and Briareos in the 22nd century, as they embark on a journey throughout dystopic ruins of New York in search of the city of Olympus. They are hired by Two Horns, the warlord of the ruined city, to eliminate bipedal combat machines. During the fight, they encounter Iris and Olson, both on a mission. Deunan and Briareos befriend them and decide to join their mission.

Iris is tasked with preventing the malevolent cyborg Talos from capturing a secret weapon humans were building. Talos uses Iris to activate the gigantic war machine.

(Source: Wikipedia)

Home to countless street acts and performances, Veludo Way attracts those interested in watching professional actors. After receiving a mysterious letter, Izumi Tachibana arrives at the venue where her father directed a once-popular theater group, Mankai Company, but learns that the building is about to be repurposed due to excessive debt. At the last minute, she convinces the debt collector to give the ensemble one more chance. He is willing to accept but on three conditions: their debut show must be successfully produced by the following month, the four all-male sub-troupes must be reinstated, and the debt must be paid off within a year. To top it off, Izumi herself must become the director. With no time to lose, Izumi quickly gathers five people for the Spring Troupe: Sakuya Sakuma, an enthusiastic high school student; Masumi Usui, a boy infatuated with Izumi; Tsuzuru Minagi, an aspiring playwright; Itaru Chigasaki, a mature office worker; and Citron, a friendly foreigner. Though they have little or no experience in acting, it's up to Izumi to train and prepare them for a performance that will restore Mankai Company to its former glory.

Medabots—powerful robots granted artificial intelligence through special "medals"—serve at the whims of their owner. They are more commonly used in "Robbatling," a popular combat sport where two medabots face off against one another. In its professional form, Medafighters use their Medabots to qualify for the World Tournament and fight amongst the elite to gain the title of champion. Elementary schooler Ikki Tenryou has just gained his first Medabot: Metabee, an outdated model with no medal. Fortunately, however, Ikki manages to find a medal in the nearby river; but when Ikki places it into Metabee's head, the latter starts to exhibit strange behaviour. Short-tempered and rebellious, he refuses to obey Ikki's orders. However, to climb the ranks to the World Tournament, Ikki and Metabee must first learn to work together, no matter how difficult the prospect may seem… [Written by MAL Rewrite]